Hormonal regulation of heme oxygenase induction in avian hepatocyte culture. Academic Article uri icon

Overview

abstract

  • The effects of various hormones were examined on the induction of heme oxygenase in monolayer cultures in chick embryo hepatocytes maintained in a chemically defined medium. Addition of insulin to the cultured cells markedly suppressed the activity of basal as well as Co2+-induced heme oxygenase. Treatment of cells with hydrocortisone also suppressed the basal enzyme activity, while the Co2+-induced enzyme activity was enhanced slightly. In contrast, triiodothyronine addition to the culture caused a slight increase of both uninduced and induced levels of the enzyme. This stimulatory effect of triiodothyronine was enhanced significantly by prolonged incubation of cells (48-96 hr) in the serum-free medium. These findings indicate that heme oxygenase synthesis can be substantially altered by changing the hormonal environment of the hepatocytes. Furthermore, the induction of heme oxygenase by Co2+ was inhibited by glucagon, dibutyryl cAMP and theophylline in a dose-dependent manner, suggesting that the enzyme induction may also be controlled by changes in cAMP levels.

publication date

  • August 15, 1985

Research

keywords

  • Heme Oxygenase (Decyclizing)
  • Hormones
  • Liver
  • Mixed Function Oxygenases

Identity

Scopus Document Identifier

  • 0021934122

Digital Object Identifier (DOI)

  • 10.1016/0006-2952(85)90019-x

PubMed ID

  • 2992523

Additional Document Info

volume

  • 34

issue

  • 16