Glycosaminoglycan antigens in peripheral nerve. Studies with antibodies from a patient with neuropathy and monoclonal gammopathy. Academic Article uri icon

Overview

abstract

  • An immunoblot staining procedure was developed for the detection of antibody binding to glycosaminoglycans (GAGs). The method was used to study the binding of a human monoclonal antibody (M-protein) from a patient with peripheral neuropathy, previously found to react with proteoglycans (PGs). GAGs prepared from human peripheral nerve were separated by electrophoresis on cellulose acetate membranes, transferred onto nitrocellulose sheets, and immunostained with the M-protein. The M-protein bound to a single GAG band with intermediate mobility which eluted with 1.25 N NaCl on ion-exchange chromatography and was chondroitinase sensitive. The M-protein appeared to bind to chondroitin sulfate containing proteoglycans in peripheral nerve.

publication date

  • July 1, 1986

Research

keywords

  • Glycosaminoglycans
  • Paraproteinemias
  • Peripheral Nerves
  • Peripheral Nervous System Diseases

Identity

Scopus Document Identifier

  • 0022491051

Digital Object Identifier (DOI)

  • 10.1016/0165-5728(86)90097-4

PubMed ID

  • 3011858

Additional Document Info

volume

  • 12

issue

  • 1