Sulfur-34S and 36S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses. Academic Article uri icon

Overview

abstract

  • Quantitative proteome profiling of microorganisms by isotopic labeling of amino acids is still a challenge, because only microorganisms with auxotrophic character are able to embed amino acids into their biomass in a quantitatively correct manner. Here, we describe an isotopic labeling technique (sulfur stable isotope labeling of amino acids for quantification, SULAQ) for the sulfur-containing amino acids cysteine and methionine in a broad range of organisms. The metabolic labeling approach is suitable for gel-based and gel-free protein analysis.

publication date

  • January 1, 2018

Research

keywords

  • Amino Acids
  • Isotope Labeling
  • Proteome
  • Proteomics
  • Sulfur Isotopes
  • Sulfur Radioisotopes

Identity

Scopus Document Identifier

  • 85054167942

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-8695-8_12

PubMed ID

  • 30259486

Additional Document Info

volume

  • 1841