In vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host-Pathogen Settings. Academic Article uri icon

Overview

abstract

  • Proteome profiling of bacteria internalized by host cells is still a challenging task, due to low amounts of bacterial proteins in host-pathogen settings and the high amounts of contaminating host proteins. Here, we describe a workflow for the enrichment of intracellular bacteria by fluorescence activated cell sorting which in combination with highly sensitive LC-MS/MS allows monitoring of about 1200 proteins from 2 to 4 × 106 internalized bacterial cells as starting material.

publication date

  • January 1, 2018

Research

keywords

  • Adaptation, Biological
  • Bacterial Proteins
  • Host-Pathogen Interactions
  • Proteome
  • Proteomics

Identity

Scopus Document Identifier

  • 85054156134

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-8695-8_15

PubMed ID

  • 30259489

Additional Document Info

volume

  • 1841