Therapeutic Drug Monitoring of Lacosamide by LC-MS/MS. Academic Article uri icon

Overview

abstract

  • High-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) has become a primary analytical methodology in therapeutic drug monitoring of antiepileptic drugs (AEDs). To demonstrate the utility of LC-MS/MS in measuring drug concentrations in serum or plasma, analysis of lacosamide (Vimpat) is discussed in this chapter. Lacosamide is an example of the newer-generation AEDs. The drug is extracted by protein precipitation and dilution of the serum specimen. A small volume of the extracted specimen is injected into a reversed-phase chromatography column, and lacosamide is identified by positive electrospray ionization (ESI) mass spectrometry in the multiple reaction monitoring (MRM) mode, which provides selectivity for quantitative analysis. A deuterated internal standard is used to correct for any loss of analyte during the process of extraction and analysis. A seven-point calibration curve and two levels of quality controls are included in each batch.

publication date

  • January 1, 2019

Research

keywords

  • Anticonvulsants
  • Chromatography, Liquid
  • Drug Monitoring
  • Lacosamide
  • Tandem Mass Spectrometry

Identity

Scopus Document Identifier

  • 85055167693

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-8823-5_7

PubMed ID

  • 30350280

Additional Document Info

volume

  • 1872