Tissue engineering the human auricle by auricular chondrocyte-mesenchymal stem cell co-implantation. Academic Article uri icon

Overview

abstract

  • Children suffering from microtia have few options for auricular reconstruction. Tissue engineering approaches attempt to replicate the complex anatomy and structure of the ear with autologous cartilage but have been limited by access to clinically accessible cell sources. Here we present a full-scale, patient-based human ear generated by implantation of human auricular chondrocytes and human mesenchymal stem cells in a 1:1 ratio. Additional disc construct surrogates were generated with 1:0, 1:1, and 0:1 combinations of auricular chondrocytes and mesenchymal stem cells. After 3 months in vivo, monocellular auricular chondrocyte discs and 1:1 disc and ear constructs displayed bundled collagen fibers in a perichondrial layer, rich proteoglycan deposition, and elastin fiber network formation similar to native human auricular cartilage, with the protein composition and mechanical stiffness of native tissue. Full ear constructs with a 1:1 cell combination maintained gross ear structure and developed a cartilaginous appearance following implantation. These studies demonstrate the successful engineering of a patient-specific human auricle using exclusively human cell sources without extensive in vitro tissue culture prior to implantation, a critical step towards the clinical application of tissue engineering for auricular reconstruction.

publication date

  • October 24, 2018

Research

keywords

  • Congenital Microtia
  • Ear Auricle
  • Ear Cartilage
  • Mesenchymal Stem Cell Transplantation

Identity

PubMed Central ID

  • PMC6200177

Scopus Document Identifier

  • 85055414763

Digital Object Identifier (DOI)

  • 10.1089/ten.tea.2007.0186

PubMed ID

  • 30356228

Additional Document Info

volume

  • 13

issue

  • 10