Autophagy in Platelets. Academic Article uri icon

Overview

abstract

  • Anucleate platelets are produced by fragmentation of megakaryocytes. Platelets circulate in the bloodstream for a finite period: upon vessel injury, they are activated to participate in hemostasis; upon senescence, unused platelets are cleared. Platelet hypofunction leads to bleeding. Conversely, pathogenic platelet activation leads to occlusive events that precipitate strokes and heart attacks. Recently, we and others have shown that autophagy occurs in platelets and is important for platelet production and normal functions including hemostasis and thrombosis. Due to the unique properties of platelets, such as their lack of nuclei and their propensity for activation, methods for studying platelet autophagy must be specifically tailored. Here, we describe useful methods for examining autophagy in both human and mouse platelets.

publication date

  • January 1, 2019

Research

keywords

  • Autophagosomes
  • Autophagy
  • Blood Platelets
  • Intravital Microscopy

Identity

PubMed Central ID

  • PMC7039316

Scopus Document Identifier

  • 85059496036

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-8873-0_32

PubMed ID

  • 30610718

Additional Document Info

volume

  • 1880