GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae. Academic Article uri icon

Overview

abstract

  • The gene encoding the galactose permease of Saccharomyces cerevisiae (GAL2) was cloned. The clone restores galactose permease activity to gal2 yeasts and is regulated by galactose in a manner similar to other GAL gene products (GAL1, -7, and -10). Experiments with temperature-conditional secretory mutants indicated that transport of the GAL2 gene product to the cell surface requires a functional secretory pathway. In addition, gene fusions were constructed between the GAL2 gene and the Escherichia coli lacZ gene. The GAL2-lacZ gene fusions code for galactose-regulated beta-galactosidase activity in yeasts. The beta-galactosidase activity was found to be membrane bound.

publication date

  • April 1, 1986

Research

keywords

  • Genes, Fungal
  • Membrane Transport Proteins
  • Saccharomyces cerevisiae

Identity

PubMed Central ID

  • PMC214594

Scopus Document Identifier

  • 0022454130

Digital Object Identifier (DOI)

  • 10.1128/jb.166.1.313-318.1986

PubMed ID

  • 3082856

Additional Document Info

volume

  • 166

issue

  • 1