Immune suppression in vivo with antigen-modified syngeneic cells. I. T-cell-mediated suppression to the terpolymer poly-(Glu, Lys, Phe)n. Academic Article uri icon

Overview

abstract

  • The palmitoyl derivative of the linear polypeptide of poly-(L-Glu-L-Lys-L-Phe)n (GLphi) can be coupled to spleen cells directly. The intravenous administration of 2 X 10(5)--3 X 10(7) GLphi-coupled syngeneic spleen cells induces GL-phi-specific suppressor T cells in C57BL/6 nonresponder mice. The suppression is antigen specific and can be detected by the inhibition of the primary GLphi plaque-forming cell response to challenge with GLphi-fowl gamma globulin. The number of inducer cells required for suppression carry less than 0.1 microgram of antigen. Spleen cells from tolerized mice can transfer suppression to normal syngeneic recipients. The suppression is cyclophosphamide sensitive and the suppressor cells bear the Thy 1.2 marker. This method of inducing antigen-specific suppressor cells may be generally applicable to other antigen systems.

publication date

  • December 1, 1978

Research

keywords

  • Antigens
  • Immune Tolerance
  • Immunosuppression Therapy
  • Peptides
  • T-Lymphocytes

Identity

PubMed Central ID

  • PMC2185101

Scopus Document Identifier

  • 0018218463

Digital Object Identifier (DOI)

  • 10.1084/jem.148.6.1539

PubMed ID

  • 309920

Additional Document Info

volume

  • 148

issue

  • 6