The Unmixing Problem: A Guide to Applying Single-Cell RNA Sequencing to Bone. Review uri icon

Overview

abstract

  • Bone is composed of a complex mixture of many dynamic cell types. Flow cytometry and in vivo lineage tracing have offered early progress toward deconvoluting this heterogeneous mixture of cells into functionally well-defined populations suitable for further studies. Single-cell sequencing is poised as a key complementary technique to better understand the cellular basis of bone metabolism and development. However, single-cell sequencing approaches still have important limitations, including transcriptional effects of cell isolation and sparse sampling of the transcriptome, that must be considered during experimental design and analysis to harness the power of this approach. Accounting for these limitations requires a deep knowledge of the tissue under study. Therefore, with the emergence of accessible tools for conducting and analyzing single-cell RNA sequencing (scRNA-seq) experiments, bone biologists will be ideal leaders in the application of scRNA-seq to the skeleton. Here we provide an overview of the steps involved with a single-cell sequencing analysis of bone, focusing on practical considerations needed for a successful study. © 2019 American Society for Bone and Mineral Research.

publication date

  • July 1, 2019

Research

keywords

  • Bone and Bones
  • Sequence Analysis, RNA
  • Single-Cell Analysis

Identity

PubMed Central ID

  • PMC6658136

Scopus Document Identifier

  • 85069794473

Digital Object Identifier (DOI)

  • 10.1002/jbmr.3719

PubMed ID

  • 31336008

Additional Document Info

volume

  • 34

issue

  • 7