Design and Production of Newcastle Disease Virus for Intratumoral Immunomodulation. Academic Article uri icon

Overview

abstract

  • Newcastle disease virus (NDV) is an avian paramyxovirus that has been extensively studied as an oncolytic agent, in addition to being an economically important pathogen in the poultry industry. The establishment of a reverse genetics system for this virus has enabled the development of genetically modified recombinant NDV viruses with improved oncolytic and immunotherapeutic properties. In this chapter, we describe the materials and methods involved in the in vitro cloning and rescue of NDV expressing murine 4-1BBL as well as the in vivo evaluation of NDV expressing 4-1BBL in a B16-F10 murine melanoma model.

publication date

  • January 1, 2020

Research

keywords

  • Genetic Engineering
  • Genetic Vectors
  • Immunomodulation
  • Newcastle disease virus
  • Oncolytic Viruses

Identity

PubMed Central ID

  • PMC7370866

Scopus Document Identifier

  • 85071780595

Digital Object Identifier (DOI)

  • 10.1002/0471142735.im2001s39

PubMed ID

  • 31486036

Additional Document Info

volume

  • 2058