Regulation of embryonic and adult neurogenesis by Ars2. Academic Article uri icon

Overview

abstract

  • Neural development is controlled at multiple levels to orchestrate appropriate choices of cell fate and differentiation. Although more attention has been paid to the roles of neural-restricted factors, broadly expressed factors can have compelling impacts on tissue-specific development. Here, we describe in vivo conditional knockout analyses of murine Ars2, which has mostly been studied as a general RNA-processing factor in yeast and cultured cells. Ars2 protein expression is regulated during neural lineage progression, and is required for embryonic neural stem cell (NSC) proliferation. In addition, Ars2 null NSCs can still transition into post-mitotic neurons, but fail to undergo terminal differentiation. Similarly, adult-specific deletion of Ars2 compromises hippocampal neurogenesis and results in specific behavioral defects. To broaden evidence for Ars2 as a chromatin regulator in neural development, we generated Ars2 ChIP-seq data. Notably, Ars2 preferentially occupies DNA enhancers in NSCs, where it colocalizes broadly with NSC regulator SOX2. Ars2 association with chromatin is markedly reduced following NSC differentiation. Altogether, Ars2 is an essential neural regulator that interacts dynamically with DNA and controls neural lineage development.

publication date

  • January 22, 2020

Research

keywords

  • Aging
  • DNA-Binding Proteins
  • Embryo, Mammalian
  • Neurogenesis
  • Transcription Factors

Identity

PubMed Central ID

  • PMC6983708

Scopus Document Identifier

  • 85078224518

Digital Object Identifier (DOI)

  • 10.1002/gene.10008

PubMed ID

  • 31969356

Additional Document Info

volume

  • 147

issue

  • 2