Phylogenomic assessment of drug-resistant Mycobacterium tuberculosis strains from Beira, Mozambique. Academic Article uri icon

Overview

abstract

  • BACKGROUND: Mozambique is a high-burden tuberculosis (TB) country where TB/HIV co-infection and drug resistant TB (DR-TB) incidence is increasing. Whole genome sequencing (WGS) comprehensively describes the molecular epidemiology of TB, allows prediction of DR-TB phenotypes, lineages strains identification and better understanding of transmission chains. OBJECTIVE: To describe genetic diversity of DR-TB Mycobacterium tuberculosis isolated in Beira, Mozambique. METHODS: Descriptive cross-sectional study with 35 M. tuberculosis isolates, resistant to at least one first-line drug on molecular drug-susceptibility tests (DST). Variant identification, DR prediction and phylogenetic analysis provided by WGS, drug-susceptibility pattern compared to line-probe assay (LPA): Genotype MTBDRTMplus and MTBDRTMsl. FINDINGS: Lineage 4 (L4) was the most prevalent: 25 (71.4%) isolates; 5 (14.3%) L1 and 5 (14.3%) L2. WGS showed 33/35 (94.3%) isolates resistant to at least one drug, two pan-susceptible isolates that were previously diagnosed as DR-TB with genotype MTBDRplus. Concordance between WGS and LPA: 88.6% for isoniazid (INH), 85.7% to rifampicin (RPM), 91.4% for quinolones and 100% to second line injectable drugs. There were three possible TB transmission chains, 10 strains showing recent transmission. CONCLUSION: WGS provided reliable information about the most frequent lineages related to DR-TB in Beira, Mozambique: L4.3 (LAM), L2 (Beijing) and L1 (EAI) and possible recent transmission chain.

authors

  • Hartman, Travis E.
  • Namburete, Evangelina Inacio
  • Dippenaar, Anzaan
  • Conceição, Emilyn Costa
  • Feliciano, Cinara
  • Nascimento, Margarida Maria Passeri do
  • Peronni, Kamila Chagas
  • Silva, Wilson Araújo
  • Ferro, Josefo João
  • Harrison, Lee H
  • Warren, Robin Mark
  • Bollela, Valdes Roberto

publication date

  • January 29, 2020

Research

keywords

  • Drug Resistance, Multiple, Bacterial
  • Mycobacterium tuberculosis
  • Tuberculosis, Multidrug-Resistant

Identity

PubMed Central ID

  • PMC9300053

Scopus Document Identifier

  • 85079274231

Digital Object Identifier (DOI)

  • 10.1086/592575

PubMed ID

  • 32063558

Additional Document Info

volume

  • 121