Monitoring abscopal responses to radiation in mice. Academic Article uri icon

Overview

abstract

  • Focal radiation therapy has the potential to generate systemic tumor-targeting immune responses so potent as to eradicate anatomically distant, non-irradiated malignant lesions, a phenomenon commonly referred to as "the abscopal response." In cancer patients, bona fide abscopal responses are rare, although the recent introduction of immune checkpoint blockers into the clinical practice has significantly increased their incidence. In rodents, abscopal responses can be conveniently modeled by establishing two, slightly asynchronous and anatomically distant subcutaneous tumors in syngeneic immunocompetent hosts, provided that the therapeutic partners of radiation potentially included in the regimen of choice do not mediate systemic anticancer effects per se. Here, we describe such method to monitor abscopal responses based on mammary carcinoma TSA cells implanted in syngeneic immunocompetent BALB/c mice. With minor variations, the same technique can be conveniently applied to a variety of transplantable mouse tumors.

publication date

  • April 15, 2019

Research

keywords

  • Mice, Inbred BALB C

Identity

Scopus Document Identifier

  • 85081006957

Digital Object Identifier (DOI)

  • 10.1016/bs.mie.2019.04.014

PubMed ID

  • 32122540

Additional Document Info

volume

  • 635