High-resolution optoacoustic imaging of tissue responses to vascular-targeted therapies. Academic Article uri icon

Overview

abstract

  • The monitoring of vascular-targeted therapies using magnetic resonance imaging, computed tomography or ultrasound is limited by their insufficient spatial resolution. Here, by taking advantage of the intrinsic optical properties of haemoglobin, we show that raster-scanning optoacoustic mesoscopy (RSOM) provides high-resolution images of the tumour vasculature and of the surrounding tissue, and that the detection of a wide range of ultrasound bandwidths enables the distinction of vessels of differing size, providing detailed insights into the vascular responses to vascular-targeted therapy. Using RSOM to examine the responses to vascular-targeted photodynamic therapy in mice with subcutaneous xenografts, we observed a substantial and immediate occlusion of the tumour vessels followed by haemorrhage within the tissue and the eventual collapse of the entire vasculature. Using dual-wavelength RSOM, which distinguishes oxyhaemoglobin from deoxyhaemoglobin, we observed an increase in oxygenation of the entire tumour volume immediately after the application of the therapy, and a second wave of oxygen reperfusion approximately 24 h thereafter. We also show that RSOM enables the quantification of differences in neoangiogenesis that predict treatment efficacy.

publication date

  • March 12, 2020

Research

keywords

  • Diagnostic Imaging
  • Neovascularization, Pathologic
  • Photoacoustic Techniques
  • Ultrasonography
  • Vascular Neoplasms

Identity

PubMed Central ID

  • PMC7153756

Scopus Document Identifier

  • 85081725975

Digital Object Identifier (DOI)

  • 10.1016/j.pacs.2017.03.001

PubMed ID

  • 32165736

Additional Document Info

volume

  • 4

issue

  • 3