Two-Step Bacterial Artificial Chromosome (BAC) Engineering: Preparation and Verification of the Recombinant Shuttle Vector. Academic Article uri icon

Overview

abstract

  • Plasmid DNA is prepared from the recombinant shuttle vector pLD53.SCAB/A-B created by cloning of the A and B homology arms for two-step bacterial artificial chromosome (BAC) engineering. To confirm that the A-box and B-box arms have been successfully incorporated into pLD53.SCAB, the pattern of enzyme digestion of the modified plasmid is compared with that of the unmodified pLD53.SCAB. Once the shuttle vector is shown to carry the proper sequences, it is ready for transfer into the BAC host.

publication date

  • April 1, 2020

Research

keywords

  • Chromosomes, Artificial, Bacterial
  • Cloning, Molecular
  • Genetic Engineering
  • Genetic Vectors

Identity

Scopus Document Identifier

  • 85082979586

Digital Object Identifier (DOI)

  • 10.1101/pdb.prot098061

PubMed ID

  • 32238592

Additional Document Info

volume

  • 2020

issue

  • 4