Amplification of altered self-reactive cytolytic T lymphocyte responses by cloned, allospecific human Th cells. Academic Article uri icon

Overview

abstract

  • The effect of a cloned allospecific human Th cell, termed 86, on the in vitro generation of altered self-reactive cytolytic T lymphocytes (CTL) was investigated. Utilizing the induction of hapten altered self-reactive CTL as a model for virus or tumor-specific cell-mediated immunity, we determined that the presence of small numbers of clone 86 cells markedly amplified the generation of hapten altered self-reactive CTL. The killer cells induced belong to the CD4-, CD8+ subset, are specific for the hapten-modified autologous stimulator cells present in culture, and are MHC class I restricted. The CTL induced under these culture conditions are readily expanded in the presence of IL-2 with maintenance of efficient and specific altered self-killing. Of interest, clone 86 cells preferentially enhance the growth of CD8+ T cells and selectively amplify altered self-cytolysis but not NK cell activity. Although in vitro clone 86 cells mediate help for CTL generation via the production of lymphokines (IL-4 but little IL-2), one can envision immunotherapeutic strategies for human disease that involve the adoptive transfer of Th cells functionally analogous to clone 86.

publication date

  • November 1, 1988

Research

keywords

  • Cytotoxicity, Immunologic
  • T-Lymphocytes, Cytotoxic

Identity

PubMed Central ID

  • PMC442743

Scopus Document Identifier

  • 0024209570

Digital Object Identifier (DOI)

  • 10.1172/JCI113786

PubMed ID

  • 3263397

Additional Document Info

volume

  • 82

issue

  • 5