Functional annotation of human long noncoding RNAs via molecular phenotyping. Academic Article uri icon

Overview

abstract

  • Long noncoding RNAs (lncRNAs) constitute the majority of transcripts in the mammalian genomes, and yet, their functions remain largely unknown. As part of the FANTOM6 project, we systematically knocked down the expression of 285 lncRNAs in human dermal fibroblasts and quantified cellular growth, morphological changes, and transcriptomic responses using Capped Analysis of Gene Expression (CAGE). Antisense oligonucleotides targeting the same lncRNAs exhibited global concordance, and the molecular phenotype, measured by CAGE, recapitulated the observed cellular phenotypes while providing additional insights on the affected genes and pathways. Here, we disseminate the largest-to-date lncRNA knockdown data set with molecular phenotyping (over 1000 CAGE deep-sequencing libraries) for further exploration and highlight functional roles for ZNF213-AS1 and lnc-KHDC3L-2.

authors

publication date

  • July 27, 2020

Research

keywords

  • RNA, Long Noncoding

Identity

PubMed Central ID

  • PMC7397864

Scopus Document Identifier

  • 85089203111

Digital Object Identifier (DOI)

  • 10.1016/j.molcel.2016.08.010

PubMed ID

  • 32718982

Additional Document Info

volume

  • 30

issue

  • 7