Time-resolved cryo-EM using Spotiton. Academic Article uri icon

Overview

abstract

  • We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study short-lived molecular states. Using piezoelectric dispensing, two independent streams of ~50-pl droplets of sample are deposited within 10 ms of each other onto the surface of a nanowire EM grid, and the mixing reaction stops when the grid is vitrified in liquid ethane ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest.

publication date

  • August 10, 2020

Research

keywords

  • Cryoelectron Microscopy

Identity

PubMed Central ID

  • PMC7799389

Scopus Document Identifier

  • 85089257100

Digital Object Identifier (DOI)

  • 10.1038/s41586-020-2116-0

PubMed ID

  • 32778833

Additional Document Info

volume

  • 17

issue

  • 9