Specimen Preparation for Single-Cell Sequencing Analysis of Skeletal Cells. Academic Article uri icon

Overview

abstract

  • Recent work emphasizes that bone comprises numerous mesenchymal cell types each with different biologic functions, and deconvoluting the functions of these cells requires technical approaches with single-cell resolution, such as single-cell RNA sequencing (scRNA-seq). A critical step in conducting a successful single-cell sequencing study of bone is generation of a single-cell suspension of skeletal cells while preserving cell viability. Here we describe a method to prepare single-cell suspensions from skeletal tissue in preparation for single-cell sequencing studies. Also included are optional steps for fluorescence-activated cell sorting (FACS) of skeletal cells, which allows subsequent scRNA-seq studies to be focused on specific populations of interest.

publication date

  • January 1, 2021

Research

keywords

  • Bone and Bones
  • Osteocytes
  • Single-Cell Analysis
  • Specimen Handling

Identity

Scopus Document Identifier

  • 85091765516

Digital Object Identifier (DOI)

  • 10.1007/978-1-0716-0989-7_7

PubMed ID

  • 32979200

Additional Document Info

volume

  • 2221