Single- and two- chain tissue type plasminogen activator treatments differentially influence cerebral recovery after stroke. Academic Article uri icon

Overview

abstract

  • Tissue type Plasminogen Activator (tPA), named alteplase (Actilyse®) under its commercial form, is currently the only pharmacological treatment approved during the acute phase of ischemic stroke, used either alone or combined with thrombectomy. Interestingly, the commercial recombinant tPA (rtPA) contains two physiological forms of rtPA: the single chain rtPA (sc-rtPA) and the two-chains rtPA (tc-rtPA), with differential properties demonstrated in vitro. Using a relevant mouse model of thromboembolic stroke, we have investigated the overall effects of these two forms of rtPA when infused early after stroke onset (i.e. 20 min) on recanalization, lesion volumes, alterations of the integrity of the blood brain barrier and functional recovery. Our data reveal that there is no difference in the capacity of sc-rtPA and tc-rtPA to promote fibrinolysis and reperfusion of the tissue. However, compared to sc-rtPA, tc-rtPA is less efficient to reduce lesion volumes and to improve functional recovery, and is associated with an increased opening of the blood brain barrier. These data indicate better understanding of differential effects of these tPA forms might be important to ultimately improve stroke treatment.

publication date

  • January 13, 2021

Research

keywords

  • Fibrinolytic Agents
  • Recovery of Function
  • Stroke
  • Tissue Plasminogen Activator

Identity

Scopus Document Identifier

  • 85100154224

Digital Object Identifier (DOI)

  • 10.1016/j.expneurol.2021.113606

PubMed ID

  • 33453214

Additional Document Info

volume

  • 338