Simultaneous Detection of Phosphoinositide Lipids by Radioactive Metabolic Labeling. Academic Article uri icon

Overview

abstract

  • Phosphoinositide (PPI) lipids are a crucial class of low-abundance signaling molecules that regulate many processes within cells. Methods that enable simultaneous detection of all PPI lipid species provide a wholistic snapshot of the PPI profile of cells, which is critical for probing PPI biology. Here we describe a method for the simultaneous measurement of cellular PPI levels by metabolically labeling yeast or mammalian cells with myo-3H-inositol, extracting radiolabeled glycerophosphoinositides, and separating lipid species on an anion exchange column via HPLC.

publication date

  • January 1, 2021

Research

keywords

  • Isotope Labeling
  • Phosphatidylinositol Phosphates
  • Phosphatidylinositols

Identity

PubMed Central ID

  • PMC8059495

Scopus Document Identifier

  • 85100324963

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2013.08.003

PubMed ID

  • 33481228

Additional Document Info

volume

  • 2251