Vaccinia virus late transcripts generated in vitro have a poly(A) head. Academic Article uri icon

Overview

abstract

  • A cell free system mediating accurate transcription of vaccinia virus genes was established using lysates of cells in the late phase of infection. Vaccinia late genes are faithfully transcribed in this extract whereas cellular pol II and pol III promoters are not recognized. The late viral transcripts contain a poly(A) head of approximately 35 nt at the 5' end which is not co-linearly encoded in the externally added template. The transcripts obtained in vitro are indistinguishable from the mature in vivo RNAs. The poly(A) head is synthesized de novo and its formation appears to be directly coupled to the transcription of the gene. The synthesis of the poly(A) head transcripts in vitro is consistent with a proposed slippage model.

publication date

  • April 1, 1988

Research

keywords

  • Genes, Viral
  • Poly A
  • Transcription, Genetic
  • Vaccinia virus

Identity

PubMed Central ID

  • PMC454454

Scopus Document Identifier

  • 0023992372

Digital Object Identifier (DOI)

  • 10.1002/j.1460-2075.1988.tb02929.x

PubMed ID

  • 3402436

Additional Document Info

volume

  • 7

issue

  • 4