A Golgi-derived vesicle potentiates PtdIns4P to PtdIns3P conversion for endosome fission. Academic Article uri icon

Overview

abstract

  • Endosome fission is essential for cargo sorting and targeting in the endosomal system. However, whether organelles other than the endoplasmic reticulum (ER) participate in endosome fission through membrane contacts is unknown. Here, we characterize a Golgi-derived vesicle, the SEC14L2 compartment, that plays a unique role in facilitating endosome fission through ternary contacts with endosomes and the ER. Localized to the ER-mediated endosome fission site, the phosphatidylinositol transfer protein SEC14L2 promotes phosphatidylinositol 4-phosphate (PtdIns4P) to phosphatidylinositol 3-phosphate (PtdIns3P) conversion before endosome fission. In the absence of SEC14L2, endosome fission is attenuated and more enlarged endosomes arise due to endosomal accumulation of PtdIns4P and reduction in PtdIns3P. Collectively, our data suggest roles of the Golgi network in ER-associated endosome fission and a mechanism involving ER-endosome contacts in the regulation of endosomal phosphoinositide conversion.

publication date

  • June 28, 2021

Research

keywords

  • Carrier Proteins
  • Endoplasmic Reticulum
  • Endosomes
  • Golgi Apparatus
  • Phosphatidylinositol Phosphates
  • Zebrafish Proteins

Identity

Scopus Document Identifier

  • 85110228680

Digital Object Identifier (DOI)

  • 10.1038/nprot.2017.065

PubMed ID

  • 34183801

Additional Document Info

volume

  • 23

issue

  • 7