Effect of multiple freeze-thaw cycles on the detection of anti-SARS-CoV-2 IgG antibodies. Academic Article uri icon

Overview

abstract

  • Several studies have investigated the effect of repeated freeze-thaw (F/T) cycles on RNA detection for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, no data are available regarding the effect of repeated F/T cycles on SARS-CoV-2 antibody detection in serum. We investigated the effect of multiple F/T cycles on anti-SARS-CoV-2 IgG detection using an ELISA test targeting the nucleocapsid antibodies. Ten positive and 1 negative SARS-CoV-2 IgG sera from 11 participants, in replicates of 5, were subjected to a total of 16 F/T cycles and stored at 4 °C until tested by ELISA. Statistical analysis was performed to test for F/T cycle effect. None of the 10 positive sera became negative after 16 F/T cycles. There was no significant difference in the OD average reading between the first and last F/T cycles, except for one serum with a minimal decline in the OD. The random effect linear regression of log (OD) on the number of cycles showed no significant trend, with a slope consistent with zero (B=-0.0001; 95 % CI -0.0008; 0.0006; P-value=0.781). These results suggest that multiple F/T cycles had no effect on the ability of the ELISA assay to detect SARS-CoV-2 IgG antibodies.

publication date

  • August 1, 2021

Research

keywords

  • Antibodies, Viral
  • Blood Preservation
  • COVID-19
  • Cryopreservation
  • Immunoglobulin G
  • SARS-CoV-2

Identity

PubMed Central ID

  • PMC8513627

Scopus Document Identifier

  • 85113318135

Digital Object Identifier (DOI)

  • 10.1016/j.ijid.2020.10.042

PubMed ID

  • 34356000

Additional Document Info

volume

  • 70

issue

  • 8