Aging, inflammation and DNA damage in the somatic testicular niche with idiopathic germ cell aplasia. Academic Article uri icon

Overview

abstract

  • Molecular mechanisms associated with human germ cell aplasia in infertile men remain undefined. Here we perform single-cell transcriptome profiling to highlight differentially expressed genes and pathways in each somatic cell type in testes of men with idiopathic germ cell aplasia. We identify immaturity of Leydig cells, chronic tissue inflammation, fibrosis, and senescence phenotype of the somatic cells, as well markers of chronic inflammation in the blood. We find that deregulated expression of parentally imprinted genes in myoid and immature Leydig cells, with relevant changes in the ratio of Lamin A/C transcripts and an active DNA damage response in Leydig and peritubular myoid cells are also indicative of senescence of the testicular niche. This study offers molecular insights into the pathogenesis of idiopathic germ cell aplasia.

publication date

  • September 1, 2021

Research

keywords

  • Aging
  • DNA Damage
  • Inflammation
  • Testis

Identity

PubMed Central ID

  • PMC8410861

Scopus Document Identifier

  • 85114175311

Digital Object Identifier (DOI)

  • 10.1093/nar/gkw377

PubMed ID

  • 34471128

Additional Document Info

volume

  • 12

issue

  • 1