Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar. Academic Article uri icon

Overview

abstract

  • Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792 nasopharyngeal PCR-positive samples collected between April-June 2021 were successfully genotyped, revealing the importation and transmission dynamics of these three variants in Qatar.

publication date

  • September 12, 2021

Research

keywords

  • COVID-19
  • SARS-CoV-2

Identity

Scopus Document Identifier

  • 85116134470

Digital Object Identifier (DOI)

  • 10.1016/j.ijid.2021.09.006

PubMed ID

  • 34525398

Additional Document Info

volume

  • 112