Reconstruction of neocortex: Organelles, compartments, cells, circuits, and activity. Academic Article uri icon

Overview

abstract

  • We assembled a semi-automated reconstruction of L2/3 mouse primary visual cortex from ∼250 × 140 × 90 μm3 of electron microscopic images, including pyramidal and non-pyramidal neurons, astrocytes, microglia, oligodendrocytes and precursors, pericytes, vasculature, nuclei, mitochondria, and synapses. Visual responses of a subset of pyramidal cells are included. The data are publicly available, along with tools for programmatic and three-dimensional interactive access. Brief vignettes illustrate the breadth of potential applications relating structure to function in cortical circuits and neuronal cell biology. Mitochondria and synapse organization are characterized as a function of path length from the soma. Pyramidal connectivity motif frequencies are predicted accurately using a configuration model of random graphs. Pyramidal cells receiving more connections from nearby cells exhibit stronger and more reliable visual responses. Sample code shows data access and analysis.

authors

publication date

  • February 24, 2022

Research

keywords

  • Neocortex

Identity

PubMed Central ID

  • PMC9337909

Scopus Document Identifier

  • 85126290259

Digital Object Identifier (DOI)

  • 10.1101/2020.03.25.007468

PubMed ID

  • 35216674

Additional Document Info

volume

  • 185

issue

  • 6