Homogalacturonan from squash: Characterization and tau-binding pattern of a sulfated derivative. Academic Article uri icon

Overview

abstract

  • A pectic polysaccharide (WAP) was isolated from squash and identified as a homogalacturonan with a molecular mass of 83.2 kDa by GPC, monosaccharide composition analysis, FT-IR and NMR spectra. Sulfation modification of WAP was carried out and a sulfated derivative (SWAP) was obtained with a substitution degree of 1.81. The NMR spectrum indicated that the sulfation modification mainly occurred at the C-2 and C-3 positions of galacturonan residues. The binding pattern of SWAP to tau K18 protein was observed in 2D 1H15N HSQC spectra of tau, which resembled the tau-heparin interaction, with R2 domain as the major binding region. These results suggest that SWAP has the potential to act as a heparin mimic to inhibit the transcellular spread of tau; thus natural polysaccharide from squash may be developed into therapies for AD and related tauopathies.

publication date

  • February 15, 2022

Research

keywords

  • Pectins
  • Sulfates

Identity

PubMed Central ID

  • PMC9482147

Scopus Document Identifier

  • 85124672646

Digital Object Identifier (DOI)

  • 10.1080/10408398.2021.1985428

PubMed ID

  • 35287864

Additional Document Info

volume

  • 285