Effects of end-stage osteoarthritis on markers of skeletal muscle Long INterspersed Element-1 activity. Academic Article uri icon

Overview

abstract

  • OBJECTIVE: Long INterspersed Element-1 (L1) is an autonomous transposable element in the genome. L1 transcripts that are not reverse transcribed back into the genome can accumulate in the cytoplasm and activate an inflammatory response via the cyclic GMP-AMP (cGAS)-STING pathway. We examined skeletal muscle L1 markers as well as STING protein levels in 10 older individuals (63 ± 11 y, BMI = 30.2 ± 6.8 kg/m2) with end-stage osteoarthritis (OA) undergoing total hip (THA, n = 4) or knee (TKA, n = 6) arthroplasty versus 10 young, healthy comparators (Y, 22 ± 2 y, BMI = 23.2 ± 2.5 kg/m2). For OA, muscle was collected from surgical (SX) and contralateral (CTL) sides whereas single vastus lateralis samples were collected from Y. RESULTS: L1 mRNA was higher in CTL and SX compared to Y (p < 0.001 and p = 0.001, respectively). Protein expression was higher in SX versus Y for ORF1p (p = 0.002) and STING (p = 0.022). While these data are preliminary due to limited n-sizes and the lack of a BMI-matched younger control group, higher L1 mRNA expression, ORF1p and STING protein are evident in older versus younger adults. More research is needed to determine whether cGAS-STING signaling contributes to heightened muscle inflammation during aging and/or OA.

publication date

  • July 7, 2022

Research

keywords

  • Long Interspersed Nucleotide Elements
  • Muscle, Skeletal
  • Osteoarthritis

Identity

PubMed Central ID

  • PMC9264706

Scopus Document Identifier

  • 85133638626

Digital Object Identifier (DOI)

  • 10.1177/1089253211436350

PubMed ID

  • 35799274

Additional Document Info

volume

  • 15

issue

  • 1