Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5' poly(A) leader. Academic Article uri icon

Overview

abstract

  • We have demonstrated by primer elongation and cap analysis that mature vaccinia virus late transcripts are discontinuously synthesized. We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the "start site" determined by S1 mapping using vaccinia genomic DNA as a probe. Sequencing of the RNA and of the first strand cDNA reveal that a homopolymeric poly(A) sequence is linked to the 5' terminus of the RNA transcripts. S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position -1, confirms the existence of a poly(A) leader of approximately 35 A-residues.

publication date

  • July 17, 1987

Research

keywords

  • Poly A
  • RNA Processing, Post-Transcriptional
  • RNA, Messenger
  • Transcription, Genetic
  • Vaccinia virus

Identity

PubMed Central ID

  • PMC7133236

Scopus Document Identifier

  • 0023658324

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(87)90212-1

PubMed ID

  • 3594569

Additional Document Info

volume

  • 50

issue

  • 2