ELISA-based quantification of type I IFN secretion by irradiated cancer cells. Academic Article uri icon

Overview

abstract

  • Cancer cell-intrinsic type I interferon (IFN-I) activation is required to initiate early innate immune responses and the subsequent radiation-induced anti-tumor immunity. Investigating the secretion of IFN-I cytokines in response to radiation therapy (RT) is therefore a critical readout for selecting the best immunogenic radiation dose-fractionation regimen. In this chapter, we present different ELISA-based quantification techniques that can be utilized to assess the secretion of tumor-derived IFN-I cytokines, namely IFN-α and IFN-β.

publication date

  • March 17, 2022

Research

keywords

  • Interferon Type I
  • Neoplasms

Identity

Scopus Document Identifier

  • 85126563604

Digital Object Identifier (DOI)

  • 10.1016/bs.mcb.2022.01.004

PubMed ID

  • 36064222

Additional Document Info

volume

  • 172