MINI: A high-throughput point-of-care device for performing hundreds of nucleic acid tests per day. Academic Article uri icon

Overview

abstract

  • There are a variety of infectious diseases with a high incidence and mortality in limited resource settings that could benefit from rapid point of care molecular diagnosis. Global health efforts have sought to implement mass-screening programs to provide earlier detection and subsequent treatment in an effort to control transmission and improve health outcomes. However, many of the current diagnostic technologies under development are limited to fewer than 10 samples per run, which inherently restricts the screening throughput of these devices. We have developed a high throughput device called "MINI" that is capable of testing hundreds of samples per day at the point-of-care. MINI can utilize multiple energy sources - electricity, flame, or solar - to perform loop-mediated isothermal amplification (LAMP) in a portable and robust device which is ideal for use in limited resource settings. The unique opto-electronic design of MINI minimizes the energy and space requirements of the device and maximizes the optical isolation and signal clarity, enabling point-of-care analysis of 96 unique samples at once. We show comparable performance to a commercial instrument using two different LAMP assays for Kaposi's sarcoma-associated herpesvirus and a common housekeeping gene, GAPDH. With a single device capable of running hundreds of samples per day, increased access to modern molecular diagnostics could improve health outcomes for a variety of diseases common in limited resource settings.

publication date

  • August 24, 2022

Research

keywords

  • Biosensing Techniques
  • Communicable Diseases
  • Nucleic Acids

Identity

Scopus Document Identifier

  • 85137176766

Digital Object Identifier (DOI)

  • 10.1016/j.bios.2022.114654

PubMed ID

  • 36084523

Additional Document Info

volume

  • 216