Genome-wide profiling of histone modifications in Plasmodium falciparum using CUT&RUN. Academic Article uri icon

Overview

abstract

  • We recently adapted a CUT&RUN protocol for genome-wide profiling of chromatin modifications in the human malaria parasite Plasmodium Using the step-by-step protocol described below, we were able to generate high-quality profiles of multiple histone modifications using only a small fraction of the cells required for ChIP-seq. Using antibodies against two commonly profiled histone modifications, H3K4me3 and H3K9me3, we show here that CUT&RUN profiling is highly reproducible and closely recapitulates previously published ChIP-seq-based abundance profiles of histone marks. Finally, we show that CUT&RUN requires substantially lower sequencing coverage for accurate profiling compared with ChIP-seq.

publication date

  • November 15, 2022

Research

keywords

  • Histone Code
  • Plasmodium falciparum

Identity

PubMed Central ID

  • PMC9670794

Scopus Document Identifier

  • 85141982161

Digital Object Identifier (DOI)

  • 10.1186/gb-2008-9-9-r137

PubMed ID

  • 36379668

Additional Document Info

volume

  • 6

issue

  • 1