Characterization of Ca2+-ATPase, LMCA1, with native cell membrane nanoparticles system. Academic Article uri icon

Overview

abstract

  • Ca2+-ATPases are membrane pumps that transport calcium ions across the cell membrane and are dependent on ATP. The mechanism of Listeria monocytogenes Ca2+-ATPase (LMCA1) in its native environment remains incompletely understood. LMCA1 has been investigated biochemically and biophysically with detergents in the past. This study characterizes LMCA1 using the detergent-free Native Cell Membrane Nanoparticles (NCMNP) system. As demonstrated by ATPase activity assays, the NCMNP7-25 polymer is compatible with a broad pH range and Ca2+ ions. This result suggests that NCMNP7-25 may have a wider array of applications in membrane protein research.

publication date

  • February 28, 2023

Research

keywords

  • Adenosine Triphosphatases
  • Calcium-Transporting ATPases

Identity

Scopus Document Identifier

  • 85150373635

Digital Object Identifier (DOI)

  • 10.1016/j.bbamem.2023.184143

PubMed ID

  • 36863681

Additional Document Info

volume

  • 1865

issue

  • 5