Environmentally Ultrasensitive Fluorine Probe to Resolve Protein Conformational Ensembles by 19F NMR and Cryo-EM. Academic Article uri icon

Overview

abstract

  • Limited chemical shift dispersion represents a significant barrier to studying multistate equilibria of large membrane proteins by 19F NMR. We describe a novel monofluoroethyl 19F probe that dramatically increases the chemical shift dispersion. The improved conformational sensitivity and line shape enable the detection of previously unresolved states in one-dimensional (1D) 19F NMR spectra of a 134 kDa membrane transporter. Changes in the populations of these states in response to ligand binding, mutations, and temperature correlate with population changes of distinct conformations in structural ensembles determined by single-particle cryo-electron microscopy (cryo-EM). Thus, 19F NMR can guide sample preparation to discover and visualize novel conformational states and facilitate image analysis and three-dimensional (3D) classification.

publication date

  • April 6, 2023

Research

keywords

  • Fluorine
  • Magnetic Resonance Imaging

Identity

PubMed Central ID

  • PMC10119980

Scopus Document Identifier

  • 85152137417

Digital Object Identifier (DOI)

  • 10.1107/S0907444909042073

PubMed ID

  • 37023263

Additional Document Info

volume

  • 145

issue

  • 15