Comprehensive Characterization of Protein Turnover by Comparative SILAC Labeling Analysis in 3T3-L1. Academic Article uri icon

Overview

abstract

  • A balance between the synthesis and degradation of proteins is referred to as protein turnover, which is crucial for cellular protein homeostasis. Proteome-wide analysis of protein turnover in adipocytes, which are well-known for their role in energy storage and their link to obesity and metabolism disorders, is yet to be conducted. Thus, with this objective in mind, our investigation utilized a comparative analysis of time-dependent SILAC labeling to assess protein turnover in 3T3-L1 adipocytes, spanning a period of 0 to 144 h. We observed that relatively faster or slower protein half-lives in several protein groups were associated with the PPARĪ³ signaling pathway, energy metabolism, extracellular matrix, ubiquitin-proteasome system, RNA splicing, Golgi complex, and lysosome. It is anticipated that these protein half-life profiles will provide greater clarity on the life cycle of adipocyte proteome and shed light on how they maintain protein homeostasis.

publication date

  • April 14, 2023

Research

keywords

  • Adipocytes
  • Proteome

Identity

Scopus Document Identifier

  • 85154063664

Digital Object Identifier (DOI)

  • 10.1021/acs.jproteome.2c00763

PubMed ID

  • 37057806

Additional Document Info

volume

  • 22

issue

  • 6