Viral Transduction of Mammalian Spermatogonial Stem Cells. Academic Article uri icon

Overview

abstract

  • Lentiviral vectors have been major tools for genetic manipulation of spermatogonial stem cells (SSCs) in vitro. Adeno-associated viral vectors are promising emerging tools for in vivo SSC transduction that are less invasive, compared to lentivirus, since AAV DNA is not integrated into the host genome and the host genome remains intact. In this chapter, we describe protocols using lentiviral and adeno-associated viral vectors to transduce SSCs in vitro and vivo, respectively.

publication date

  • January 1, 2023

Research

keywords

  • Genetic Techniques
  • Mammals

Identity

Scopus Document Identifier

  • 85160873699

Digital Object Identifier (DOI)

  • 10.1038/s41598-019-49624-w

PubMed ID

  • 37249874

Additional Document Info

volume

  • 2656