Topical ozone accelerates diabetic wound healing by promoting re-epithelialization via activation of IGF1R-EGFR signaling. Academic Article uri icon

Overview

abstract

  • Ozonated oil (O3) increases healing of chronic diabetic wounds, but the underlying mechanisms remain unclear. We investigated the effect of topical O3 on wound healing in diet-induced obese (DIO) diabetic mice and further elucidate the role of epidermal growth factor receptor (EGFR) and insulin-like growth factor-1 receptor (IGF1R) signaling in diabetic wound healing. We found that topical ozonated oil accelerated wound healing, increased phosphorylation of IGF1R, EGFR, and vascular endothelial growth factor receptor, and improved vascularization at the wound leading edge in DIO diabetic mice. Exposure of normal epidermal keratinocytes to ozonated medium (20μM for 2 hours daily) increased cell proliferation and migration distance through increasing phosphorylation of IGF1R and EGFR and downstream phosphoinositide 3-kinase (PI3K), Protein kinase B or Akt (PKB/Akt), and extracellular signal-regulated kinase (ERK). These findings shed light on the mechanism for topical ozone action in chronic wounds and support its potential therapeutic application.

publication date

  • June 7, 2023

Identity

Digital Object Identifier (DOI)

  • 10.1016/j.jid.2023.05.015

PubMed ID

  • 37295490