Ganglioside biosynthesis in rat liver. Characterization of three sialyltransferases. Academic Article uri icon

Overview

abstract

  • Three sialyltransferase activities involved in ganglioside biosynthesis were studied in Golgi-enriched preparations of rat liver: the formation of GM3, GD3 and GD1a. The conditions for the quantitative assays of these enzymatic reactions were standardized and optimized, with Triton X-100 being used as detergent. The apparent Km values of each sialyltransferase for N-acetyl-2-(5'-cytidylyl)neuraminic acid (1.5 mM with GM3 synthase, 0.2 mM with GD3 synthase, and 0.5 mM with GD1a synthase) and the respective glycolipid substrates (0.08 mM for lactosylceramide, 0.1 mM for GM3, and 0.5 mM for GM1) were determined. Competition experiments showed that the three sialyltransferase activities are three individual catalytic entities. Moreover, evidence was found that product inhibition may play a role in the regulation of the activity of sialyltransferases.

publication date

  • October 1, 1986

Research

keywords

  • Gangliosides
  • Liver
  • Sialyltransferases

Identity

Scopus Document Identifier

  • 0023039167

Digital Object Identifier (DOI)

  • 10.1111/j.1432-1033.1986.tb09934.x

PubMed ID

  • 3769920

Additional Document Info

volume

  • 160

issue

  • 1