Radioimmunoassay of rat apolipoprotein A-IV. Academic Article uri icon

Overview

abstract

  • We have developed a specific and sensitive radioimmunoassay for rat apolipoprotein A-IV (apoA-IV). The protocol includes treatment of the samples for 1 h at 60 degrees C with 0.7% Tween 20. Under these conditions, linear logit-log plots have been obtained for apoA-IV in lymph and plasma lipoprotein fractions as well as for purified apoA-IV. The sensitivity of the assay is to 20 ng. Absolute mass values obtained with the assay were validated by comparison with values obtained with an independent method of colorimetric reading of apoA-IV separated by polyacrylamide gel electrophoresis from plasma high density lipoproteins. The concentration of apoA-IV in fasting plasma averaged 10.2 mg/dl and in the mesenteric duct lymph 15.8 and 12.6 mg/dl during the fasting and the fat absorption states, respectively.

publication date

  • November 15, 1986

Research

keywords

  • Apolipoproteins A
  • Radioimmunoassay

Identity

Scopus Document Identifier

  • 0022866789

Digital Object Identifier (DOI)

  • 10.1016/0003-2697(86)90312-x

PubMed ID

  • 3813005

Additional Document Info

volume

  • 159

issue

  • 1