Human renal cell carcinoma xenograft: morphology, growth and chemosensitivities. Academic Article uri icon

Overview

abstract

  • A primary human renal clear cell carcinoma has been developed as a xenograft (JDF-1). Passage 7 of the JDF-1 tumor retained the microscopic morphology of the primary tumor, electron micrographs have confirmed its epithelial characteristics and karyotyping of a subsequent passage has proved it is not a murine hybrid. Immunoperoxidase studies using a panel of murine monoclonal antibodies demonstrate an antigenic phenotype specific for human renal cancer. In vitro chemosensitivities of the JDF-1 tumor were determined by the double-layer soft-agar clonogenic assay method. JDF-1 showed no significant sensitivities to several standard chemotherapeutic agents, but alpha-2-interferon and difluoromethylornithine in combination synergistically inhibited its growth by 74 per cent.

publication date

  • September 1, 1985

Research

keywords

  • Carcinoma, Renal Cell
  • Kidney Neoplasms

Identity

Scopus Document Identifier

  • 0022410677

Digital Object Identifier (DOI)

  • 10.1016/s0022-5347(17)47302-2

PubMed ID

  • 3928905

Additional Document Info

volume

  • 134

issue

  • 3