Left-handed DNA and the synaptic pairing reaction promoted by Ustilago rec1 protein. Academic Article uri icon

Overview

abstract

  • Left-handed Z-DNA binds tightly to Ustilago rec1 protein. The binding reaction is strongly dependent on ATP, but complexes formed are rapidly dissociated by ADP. The parallel between the kinetics of Z-DNA binding and the synaptic pairing reaction leading to paranemic joint molecules suggests that formation of nascent heteroduplex structures in recombination is coupled with formation of left-handed Z-like DNA on the protein. Equilibrium and kinetic studies show that rec1 protein appears to have a strong Z-DNA binding site that binds Z-DNA 75 times tighter than the B form of the DNA. We propose that DNA with a structure approximated best by a left-handed Z-DNA conformation is a key intermediate in homologous pairing promoted by rec1 protein.

publication date

  • January 1, 1985

Research

keywords

  • Basidiomycota
  • DNA
  • Fungal Proteins
  • Ustilago

Identity

Scopus Document Identifier

  • 0021984352

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(85)90317-4

PubMed ID

  • 3967291

Additional Document Info

volume

  • 40

issue

  • 1