Thermal denaturation of native striatal tyrosine hydroxylase: increased thermolability of the phosphorylated form of the enzyme. Academic Article uri icon

Overview

abstract

  • Tyrosine hydroxylase was purified from bovine corpus striatum. The native enzyme had a half-life of 15 +/- 3 min at 50 degrees C. Phosphorylation of tyrosine hydroxylase with protein kinase purified from both corpus striatum and heart activated the enzyme, but activity was rapidly lost with additional preincubation of the enzyme at 30 degrees C. Thermal denaturation studies indicated that phosphorylated tyrosine hydroxylase had a half-life of 5 +/- 2 min at 50 degrees C

publication date

  • February 1, 1981

Research

keywords

  • Corpus Striatum
  • Tyrosine 3-Monooxygenase

Identity

Scopus Document Identifier

  • 0019349702

Digital Object Identifier (DOI)

  • 10.1111/j.1471-4159.1981.tb01641.x

PubMed ID

  • 6109765

Additional Document Info

volume

  • 36

issue

  • 2