A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery. Academic Article uri icon

Overview

abstract

  • The gene prlA codes for a factor that appears to function in the export of proteins in Escherichia coli. This conclusion is based on the finding that mutations altering the prlA gene product restore export of envelope proteins with defective signal sequences. Previous results showed that the prlA gene lies in an operon (spc) known to code for ten different ribosomal proteins. Our studies show that the prlA gene lies promoter-distal to the last known ribosomal protein gene in this operon. Evidence from gene fusions constructed in vitro suggests that prlA codes for a protein containing at least 300 amino acids. Thus a heretofore unidentified protein specified by a gene within the spc operon appears to be a component of the cellular protein export machinery.

publication date

  • November 1, 1982

Research

keywords

  • Bacterial Proteins
  • Escherichia coli
  • Genes
  • Genes, Bacterial
  • Operon

Identity

Scopus Document Identifier

  • 0020361434

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(82)90422-6

PubMed ID

  • 6297749

Additional Document Info

volume

  • 31

issue

  • 1