Topological linkage of circular DNA molecules promoted by Ustilago rec 1 protein and topoisomerase. Academic Article uri icon

Overview

abstract

  • We studied the formation of linked circular DNA molecules promoted by the combined action of rec 1 protein and type I topoisomerase of Ustilago maydis. When ATP was added as cofactor to reactions containing rec 1 protein, pairs of homologous circular DNA molecules became linked after addition of topoisomerase. Closed circular duplex molecules could be joined at homologous sites with circular single-stranded molecules or with other circular duplex molecules, provided that homologous single-stranded DNA fragments or RNA polymerase and nucleoside triphosphates were also added. Complexes formed were topologically linked through regions of heteroduplex DNA. When the analog adenylyl-imidodiphosphate was substituted for ATP, nonhomologous pairs of circular DNA molecules became linked.

publication date

  • October 1, 1983

Research

keywords

  • Basidiomycota
  • DNA Topoisomerases, Type I
  • DNA, Circular
  • Fungal Proteins
  • Ustilago

Identity

Scopus Document Identifier

  • 0020842566

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(83)90549-4

PubMed ID

  • 6313215

Additional Document Info

volume

  • 34

issue

  • 3