D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport. Academic Article uri icon

Overview

abstract

  • We have isolated mutants defective in high-affinity D-ribose transport. The mutations map in rbsT or rbsB , the structural gene for ribose binding protein. rbsT consists of at least one gene coding for a protein required for high-affinity transport. The high-affinity transport-defective mutants were able to utilize D-ribose, indicating that at least a second, low-affinity transport system for D-ribose is present in Escherichia coli K-12. rbsT and rbsB are located at min 84 on the E. coli genetic map and, together with rbsK , the gene coding for ribokinase , constitute an rbs operon. The order of genes is rbsP /O rbsT rbsB rbsK . The rbs operon is subject to negative control by the product of the rbsR gene. rbsR is located distal to the rbs operon and appears to form a separate transcriptional unit.

publication date

  • May 1, 1984

Research

keywords

  • Escherichia coli
  • Escherichia coli Proteins
  • Genes, Bacterial
  • Genes, Regulator
  • Operon
  • Periplasmic Binding Proteins
  • Phosphotransferases (Alcohol Group Acceptor)
  • Ribose

Identity

PubMed Central ID

  • PMC215481

Scopus Document Identifier

  • 0021364453

Digital Object Identifier (DOI)

  • 10.1128/jb.158.2.665-673.1984

PubMed ID

  • 6327616

Additional Document Info

volume

  • 158

issue

  • 2