Fatty acid-induced modifications of mouse mammary alveolar lesions in organ culture. Academic Article uri icon

Overview

abstract

  • A newly developed adult RIII mouse mammary gland organ culture system was used to examine the effects of fatty acids and a prostaglandin synthase inhibitor on the survival and/or growth of lactogenic hormone-independent mammary alveolar lesions (MAL). The number of MAL per gland that persisted in the absence of lactogenic hormones was increased in cultures treated with arachidonic acid (24.0 +/- 3.4), and was decreased after treatment with stearic acid (4.0 +/- 3.4) or indomethacin (5.0 +/- 0.6). Arachidonic acid also induced higher incorporation of [3H]thymidine into the cellular DNA of MAL containing glands. A comparison of [3H]arachidonic acid uptake into intact (MAL containing) glands and parenchyma-free fat pads revealed a selectively higher incorporation of the labeled fatty acid in the intact glands. Arachidonic acid exposure produced higher cumulative amounts (4.02 ng) of prostaglandin E2 (PGE2) which was significantly inhibited (1.66 ng) by indomethacin (p less than 0.001). In contrast, exposure to stearic acid did not lower PGE2 levels below that of controls. A concomitant increase in MAL number and PGE2 production by arachidonic acid and a decrease in both caused by indomethacin suggest that arachidonic acid conversion to PGE2 may have a facilitative role in the survival of MAL.

publication date

  • September 1, 1984

Research

keywords

  • Fatty Acids
  • Mammary Neoplasms, Experimental
  • Precancerous Conditions

Identity

Scopus Document Identifier

  • 0021175743

Digital Object Identifier (DOI)

  • 10.1093/carcin/5.9.1123

PubMed ID

  • 6432354

Additional Document Info

volume

  • 5

issue

  • 9