Rapid screening of monoclonal antibodies: new 'microstick' radioimmunoassay.
Academic Article
Overview
abstract
A new system for assaying monoclonal antibodies consisting of an 8 x 12 array of sticks which fits into a 96-well microtiter plate is described. Tests using virus specific monoclonal antibodies and virus proteins demonstrated sensitivity equivalent to the conventional microtiter plate assay. Antibody production, antigen specific antibody, and immunoglobulin isotypes could be measured under sterile conditions directly in the original fusion mixture wells and with much greater rapidity than with the microtiter plate assay.