Alterations of mammalian oocyte meiosis I with divalent cations and calmodulin. Academic Article uri icon

Overview

abstract

  • Experiments using a Ca2+/Mg2+, serum free media were carried out aimed at clarifying proposed effects of these divalent cations on in vitro meiotic maturation of mouse and cow oocytes. Agents known to perturb intracellular Ca2+ or calmodulin were also studied. Total absence of both cations restricts both oocyte species from completing meiosis I. Media containing Mg2+ and no Ca2+ permitted some maturation in both species. Absence or small amounts of Mg2+ in the media containing control amounts of Ca2+ was much more inhibitory for the cow than the mouse oocyte. Studies of mouse oocyte maturation with Verapamil, Epinephrine and A23187 demonstrated an inhibition of maturation perhaps by the intracellular Ca2+ changes these agents are alleged to induce. A dependency of mouse oocyte maturation on active Ca-Calmodulin complexes was suggested by the calmodulin inhibitor studies.

publication date

  • May 1, 1982

Research

keywords

  • Calcium-Binding Proteins
  • Calmodulin
  • Cations, Divalent
  • Meiosis
  • Oocytes
  • Ovum

Identity

Scopus Document Identifier

  • 0019969852

Digital Object Identifier (DOI)

  • 10.1016/0143-4160(82)90011-2

PubMed ID

  • 6889466

Additional Document Info

volume

  • 3

issue

  • 2